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The 24th International Symposium on Separation Sciences (ISSS2018) which was held jointly with the 21st International Conference of Analytical Methods and Human Health (AMHH) was held in Jasna, Slovakia on June 17–20, 2018. The main organizer of the event was the Department of Analytical Chemistry, Faculty of Natural Sciences of Comenius University in Bratislava, Slovakia in cooperation with the Central European Group for Separation Sciences, the Expert Group for Chromatography and Electrophoresis of the Slovak Chemical Society, the European Separation Science Society and the Slovak Vacuum Society.