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Single molecules play an important role in biology, since most cells contain one or two copies of genomic DNA molecules, which eventually determine the fate and function of the organism. This focused meeting aims to bring together researchers, who study biomolecules in silico with molecular simulation and researchers who use lab-based single-molecule methods in vitro and in vivo. Topics include: Single molecule methods Molecular dynamics simulation Molecular modelling Multiscale modelling Fluorescence Force spectroscopy Super-resolution imaging RNA structure Protein structure