ИСТИНА

It is well known that high auxin concentration inhibits root growth, but lower ones can change plant root developmental program. PIN transporters that are responsible for the auxin transport and its redistribution certainly are involved in these phenomena. However it is not so clear what do affect auxin transporters? Last decade significance of the CLE peptides especially in plant root growth and development has been shown in plenty of scientific papers. The role of the first one established CLE-like peptide - CLV3p is clear in shoot apical meristem maintenance, however the mechanisms why it stops the root growth are not so clear yet. We have tested the well-established concentration of 1μM chemically synthesized dodecaepeptide CLV3-analog on distribution and fluorescent intensity of the PIN1:GFP, PIN2:GFP, PIN3:GFP in the primary root of the Arabidopsis. We have observed that CLV3 dodecapeptide do affect PINs. In the case of PIN1:GFP we have found redistribution of the fluorescent intensity from the stellar tissue to the meristematic and root elongation zones. The fluorescence of the PIN2:GFP was less prompted after peptide treatment. The most striking effect of CLV3p was on the PIN3:GFP mutants in the meristematic zone. We can conclude that the well known auxin-dependent inhibition of root growth and development may be caused by the CLV3 dodecapeptide influence on PINs redistribution.