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UV radiation causes a significant influence on human health and nature ( UNEP, 2011). Recently a new approach has been developed, which allows a user to estimate different kinds of biologically active UV radiation (vitamin D, eye damage, erythema) using an accurate radiative transfer model based on 8-stream discrete ordinate method over the territory of Northern Eurasia and to characterize their favourable or detrimental effects on people health (Zhdanova, Chubarova, 2013). The simulations have been fulfilled for real climatological fields of aerosol, ozone, surface albedo and cloudiness with 1x1 degree resolution. A new method was recently developed for estimating the threshold on harmful effects of eye damaging UV radiation. Its application in the UV calculated scheme has revealed significant (50%) reduction of the area with UV optimum conditions in some months of the year (April, May). In addition, a new altitude UV parameterization has been developed, which accounts not only for the changes in atmospheric pressure but also for aerosol and surface albedo variations. This provides more rigorous estimates of UV irradiance and UV resources at a particular location in mountainous regions, where one degree resolution is not acceptable. All the methods were incorporated in the interactive program available via www.momsu.ru/uv/, which allows a user to calculate the UV effects over a particular point at the territory of Northern Eurasia. A special attention is paid to long-term variability of biologically-active UV radiation. Using satellite and ground-based measurements as well as UV reconstruction model we analyzed temporal variability of biologically active UV radiation on the example of Moscow region. The application of the UV reconstruction model provides the assessment of the role of different atmospheric factors in UV variability and their possible connection with global processes.