ИСТИНА

The telomere length and structure maintenance is one of the programs involved in genome stability providing systems. The one of the major participant of the DNA repair system is PARP1. PARP1 is activated by DNA breaks and involved in protein activity and specificity regulation by the posttranslational modification. PARP activity has been implicated in many aspects of genome integrity and cell survival regulation, like repair, transcription, DNA replication, cellular differentiation, mitosis and mitotic organization, cell death pathways, vesicle trafficking and telomere length regulation. It is known that telomere proteins TRF1 and TRF2 interact with PARP family proteins and controversial data about telomere length changes caused by PARP inhibition were obtained by independent investigations. We generated cell lines with stable expression of Parp1 and stable expression of short hairpin RNA counteracts PARP1 mRNA. The PARP1 influences to telomere length and structure, telomerase activity and shelterin components were revealed.