ИСТИНА

Exosomes are extracellular vesicles of 30 to100 nm in diameter, which are secreted by various cells of mammals. It is known that exosomes represent a specific way of cell-to-cell communication. Mechanisms of secretion and uptake of these vesicles are not clear. To address this problem, we fused the transmembrane protein CD63, a known exosomal marker, at its N-termini to the EGFP protein. We have shown that these proteins exit cells as a part of exosomes, substantiated by the fluorescent nanoparticle tracking analysis. Analysis also showed that there are two prevail exosome populations in general population of vesicles. The data demonstrate that the labelling of exosome with fluorescent proteins fused to CD63 could be successfully used for a live imaging of these cellular organelles