ИСТИНА

Highly stable liposomes were developed by coating phosphatidylcholine liposomes with amphiphilic N-(2-hydroxypropyl)methacrylamide copolymer. Two approaches in the preparation of coated liposomes were employed: the copolymer was added during (in prep) or after (ex post) the liposome formation. The influence of polymer concentration and coating method were evaluated using the cryoTEM, DLS and SAXS techniques. The in prep modification significantly increases, up to four weeks, the stability of liposomes against aggregation and makes the liposomal membrane non permeable towards an inorganic salt. Such enhanced longevity is attributed to the different structure of in prep coated liposomal membranes proved by SAXS technique. More detailed investigation of the internal structure of liposomal nanocontainers coated with amphiphilic HPMA-based copolymer was performed by SANS. The combination of SAXS and SANS data provided an opportunity to describe the liposomal membrane in detail.