ИСТИНА

During sterilization by electron beam ozone is formed in the air, which increases the efficiency of microorganism suppression. When studying the processes of radiation sterilization, it is necessary to estimate the contribution of the chemical factor of ozone formation. The results of experiments on the irradiation of several cultures of microorganisms are presented, which make possible to evaluate the degree of influence of the ozone formation factor in air radiolysis. In experiments, the dependence of the degree of microbial suppression on the method of installing a test tube with a nutrient medium under a beam of an accelerator was investigated. Irradiation was carried out with horizontal and vertical installation of test tubes with cultures. With the horizontal, the size of the area of contact between the medium and the ozonized air inside the tube is higher than in the vertical tube. The effect of irradiation with a horizontal position of the tube is higher than with the vertical arrangement. The irradiation process was monitored by film dosimeters located near and inside the test tubes with a substrate analog. The calculations of the amount of ozone produced in the experiments were performed, and simulation of the irradiation process was carried out using the computer code "Beam Scanning". From the results of this study we conclude that ozone formation has a significant effect on the suppression of microorganisms during irradiation.