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Non-photochemical quenching mechanisms play an important role in the optimization of the photosynthetic apparatus and plant protection against light stress. The separation of the contributions of different mechanisms in the overall level of non-photochemical quenching is an actual task of biophysics of photosynthesis. The aim of this work is to study the nature of the changes in the non-photochemical fluorescence quenching of plant leaves grown at different illumination intensities. The object of the study is Tradescantia fluminensis L. plant grown at light intensities of 50-125 μE*m-2*s-1 (low) and 875-1000 μE*m-2*s-1 (high).