ИСТИНА

DDx3 helicase belongs to the family of DEAE-box and play a key role in RNA metabolism.  A role of DDx3 helicase in HIV life cycle has been proposed as a shuttling protein responsible for the export of unspliced/partialy spliced HIV RNAs from nucleus to cytoplasm. The knockdown of DDx3 is associated with the inhibition of HIV replication. DDx3 also play a key role in the replication of Dengue, West-Nile, Hepatitis C, and Japanese Encephalitis viruses. On this basis, DDx3 helicase can be conceded as a target for developing DDx3 inhibitors to block HIV replication. Herein, we present data on expression, purification and some properties of human DDx3 helicase.  Based on diarylurea-, triazolotriazine- and pyrimidinacetamide derivatives we synthesized 43 novel compounds as inhibitors of ATPase and helicase activities of DDx3. Several compounds displayed also the activity against HIV and the tick-borne encephalitis virus replication in cell cultures.