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The degree of the association of the dye of the fluorescent family (eosin) in aqueous pool of reverse micelles and in the water solutions was determined, was analyzed and was compared. The process of the binding between eosin’s molecules and reverse micelles were studied and the energy of the binding between molecules of eosin in the water and micelles’ solution was calculated and was examined. The dependences of absorption spectra of micelles’ solutions, which consists of AOT/heptane/eosin water solution, were investigated. The degree of hydration on temperature were analyzed.