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During the last decades the hierarchical model of analyzing the factors which are influenced on the efficiency of work activity and well-being of the staff in modern enterprises. It becomes to o be one of the most adequate methodological base for realizing the principles of systemic approach to diagnostics and prevention such negative phenomenon as organizational stress, reducing of job motivation, increased staff turnover, stable rejections of innovations, distortions of social norms of interactions in the work collective, etc. (Leonova, 2014; Shein, 2010; Zankovsky, 2008). As the basic structural components this model include 3 main levels for analyzing the factors and their negative consequences which could provoke the development of intensive stress reactions at different categories of employees in organizations: (1) “a single worker” performing tasks in a particular workplace; (2) “a specialist” as a member of the concrete working group; (3)” an employee” as a member of the organizational staff in a whole. In addition, this model is focused on revealing the structural relationships between the different positions occupied by employees in organizations. It can be realized by using the set of adequate diagnostic tests and procedures of multidimensional statistical data processing. This provides the formation of an adequate database for preparing and implementing in practice the relevant set psychoprophylactic and correctional programs for different categories of organizational staff (Leonova, 2002, 2012). For using the described three-level’s model of organizational stress’ analyzing in applied research, we have elaborated two complex diagnostic-preventive systems: “Integral diagnosis and correction of stress” (MSS) and “Individual assessment of stress resistance” (IASR) (Leonova, 2004, 2011, 2017). Both of these systems are verified and now are widely used in applied psychological researches. The total sample of standardization included more than 1400 respondents, the staff of 18 different enterprises. The perspectives of practical applications of these instruments will be presented upon the examples of analyzing the specific syndromes of organizational stress in office services and virtual organizations.