ИСТИНА

Today, there are a large number of different physiologically active substances. However, most of them may never become drugs. One of the most common reasons that prevent the use of sometimes the most active compounds is the presence of undesirable side effects. Another equally important problem is related to the inability of many drugs to penetrate into the affected cells and tissues that are subject to therapeutic treatment. One of the urgent problems at present is the development of stimulus-sensitive nanocontainers as a system for delivering cytostatics to the human body during chemotherapeutic treatment of cancer. To solve this problem, it is suitable to obtain and study the properties of stimulus-sensitive microgel complexes based on N-isopropylacrylamide, 3- (N, N-dimethylaminopropyl) methacrylamide, N, N-methylenebis-acrylamide, and lecithin and phosphotidylserine. The use of liposomes alone as a delivery vehicle has its drawbacks: they are not stable enough and are quickly cleared from the body by macrophages. For this reason, it is not possible to direct medicinal substances precisely to the organs and tissues where the pathological process takes place. Microgels are a new class of polymer nanoparticles, especially interesting as delivery vehicles, as they are in the water in a swollen state and can hold various drugs inside themselves, from low molecular weight to biopolymers: proteins and nucleic acids. In addition, the use of appropriate monomers makes it possible to impart necessary properties to microgels, including such as thermal and pH sensitivity. When the pH collapses and the thermosensitive microgel, the contents of adsorbed liposomes will be released into the external solution. The dynamic light scattering method was used to study the effect of the size of liposomes and their charge density on the properties of their complexes with a microgel, the stability of the complexes at different ionic forces. Using fluorescence spectrophotometry, the kinetics of the release of liposome contents at a temperature corresponding to the collapse of the microgel was investigated.