ИСТИНА

High-performance Chelation Ion Chromatography (HPCIC) has gained a strong interest of the researchers in the last decades because of its high selectivity towards specific metal ions and expanded possibilities for analysis of complex samples. Currently, the main focus in the development of HPCIC is related to the preparation of new efficient and selective complexing stationary phases. This paper will review recent results on preparation and characterisation new chelating adsorbents and their application for chromatographic separation of metals. The significant improvement in column efficiency was achieved by using either organopolymer or silica based porous monolithic columns or by using columns packed with core-shell silica particles of 1.7 µm size. In the latter the record separation efficiency of 200,000 theoretical plates per meter was noted. The attenuation of separation selectivity remains the key priority in the development of chelating stationary phases. The role of immobilisation chemistry on selectivity of chelating ion-exchangers will be discussed for iminodiacetic acid (IDA) as a model grafted ligand. The new selectivity of chelating ion-exchangers can be obtained by immobilization of ligands having with both carboxylic and phosphonic acid functional groups in molecules. The application of diamond based stationary phases will be discussed. The possibilities of new stationary phases for the direct (without dilution) analysis of alkaline-earth and transition metals in various complex samples including seawater, mussel tissues and calcite shells of marine microorganisms will be discussed.