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Several toxins, which could be in different contents and concentrations, must be detected for quality food control. The analysis of food contaminants like mycotoxins, pesticides, antibiotics must be in global scale with high-throughput screening (HTS). The methods of assays at this time are gas and liquid-chromatography mass spectrometry and immunoassays, mainly enzyme-linked immunosorbent assays (ELISA), Lateral Flow Immunochromatographic Assay (strip-test) and Fluorescence Polarization Immunoassay (FPIA). The advantages and limitations for applications of these methods for simultaneous detection of several toxins in food will be presented and discussed. Acknowledgements: The research was supported by Grant of Russian Foundation for Basic Research (15-53-46015) “Development of a new immunoassay test-methods for determination of several mycotoxins - aflatoxins, ochratoxin A, deoxynivalenol and Fumonisin B1”