ИСТИНА

The immunochemical methods based on the specific and high affinity antibodies are widely used for high-throughput screening (HTS) and monitoring of many physiologies active compounds. At first it is important for detection of poisons and toxicants like: new synthetic abused drugs, food mycotoxins, heather metals, explosive and etc. The well-known ELISA one of them also more and more modified. Recent trend for development of new immunochemical methods will be presented and discussed. More details could be found in our recent publications [1-6] and this year reviews [7-15]. Acknowledgement This research was supported by the Federal Target Program “Scientific and scientific-pedagogical personnel of innovative Russia in 2009-2013” (State Contract № 16.740.11.0158, September 2, 2010) and Russian Foundation for Basic Research grant 11-04-91189_NSFC_а «Mechanism of the enantio-selective molecular recognition between chiral hapten and antibody: ofloxacin as model». References: 1. Qiang Wang, Simon A. Haughey, Yuan-Ming Sun, Sergei A. Eremin, Zhen-Feng Li, Hui Liu, Zhen-Lin Xu, Yu-Dong Shen, Hong-Tao Lei. Development of a fluorescence polarization immunoassay for the detection of melamine in milk and milk powder. Anal Bioanal Chem, 399(6), 2275-2284 (2011). 2. L. Eun H. Choi, Dong M. Kim, Sung-Wook Choi, Sergei A. Eremin and Hyang S. Chun. Optimisation and validation of a fluorescence polarisation immunoassay for rapid detection of zearalenone in corn. Inter. J Food Science & Tech. Vol. 46(10), 2173–2181 (2011). 3. Hongtao Lei, Gang Xue, Chunfai Yu, Simon A. Haughey, Sergei A. Eremin, Yuanming Sun, Zhanhui Wang, Zhenlin Xu, Hong Wang, Yudong Shen and Qing Wu. Fluorescence polarization as a tool for the detection of a widely used herbicide, butachlor, in polluted waters. Anal. Methods, 3, 2334-2340 (2011). 4. Zhen-Lin Xu, Qiang Wang, Hong-Tao Lei, Sergei A. Eremin, Yu-Dong Shen, Hong Wang, Ross C. Beier, Jin-Yi Yang, Ksenia A. Maksimova, Yuan-Ming Sun. A simple, rapid and high-throughput fluorescence polarization immunoassay for simultaneous detection of organophosphorus pesticides in vegetable and environmental water samples. Anal. Chim. Acta, 708(1-2), 123-129 (2011). 5. Campanella, S. Eremin, D. Lelo, E. Martini, M. Tomassetti. Reliable new immunosensor for atrazine pesticide analysis. Sens. Actuators, B: Chem., 156(1), 50-62 (2011). 6. Alexandra P. Bondarenko and Sergei A. EREMIN. Development and optimization of Fluorescence Polarization Immunoassay for detection of mycotoxins Zearalenone and Ochratoxin A in corn samples. J. Anal. Chem., 67(9), 1-6 (2012). 7. Henna Päkkilä and Tero Soukka. Simple and inexpensive immunoassay-based diagnostic tests. Bioanalytical Reviews, Volume 3, Number 1, 27-40, DOI: 10.1007/s12566-011-0021-6 (2012). 8. J. Sarah Caygill, Frank Davis, Seamus P.J. Higson. Current trends in explosive detection techniques. Talanta, 88, 14-29 (2012). 9. Aldo Roda and Massimo Guardigli. Analytical chemiluminescence and bioluminescence: latest achievements and new horizons. Anal. Bioanal. Chem., 402(1), 69-76 (2012). 10. Cristina R. Ispas, Georgeta Crivat & Silvana Andreescu. Review: Recent Developments in Enzyme-Based Biosensors for Biomedical Analysis. Anal. Lett. 45(2-3), 168-186 (2012). 11. Goretti Díaz-Díaz, Daniel Antuña-Jiménez, M. Carmen Blanco-López, M. Jesús Lobo-Castañón, Arturo J. Miranda-Ordieres, Paulino Tuñón-Blanco. New materials for analytical biomimetic assays based on affinity and catalytic receptors prepared by molecular imprinting. Trends in Anal. Chem., Vol. 33, 68-80 (2012). 12. Rastislav Monosik, Miroslav Stredansky, Jan Tkac and Ernest Sturdik. Application of Enzyme Biosensors in Analysis of Food and Beverages. Food Anal. Meth., 5(1), 40-53 (2012). 13. Samantha P. Stewart, Steven E.J. Bell, Nicholas C. Fletcher, Samira Bouazzaoui, Yen Cheng Ho, S. James Speers, K. Laota Peters. Raman spectroscopy for forensic examination of β-ketophenethylamine “legal highs”: Reference and seized samples of cathinone derivatives. Anal. Chim. Acta, 712, 1-6 (2012). 14. Stacy E.F. Melanson, Marion L. Snyder, Petr Jarolim, and James G. Flood. A New Highly Specific Buprenorphine Immunoassay for Monitoring Buprenorphine Compliance and Abuse. J. Analyt. Toxicol., 36(3), 201-206 (2012). 15. Zentai A., Sali J., Szeitzné-Szabó M., Szabó I.J., Ambrus Á. Exposure of consumers to morphine from poppy seeds in Hungary. Food Addit. Contam, 29(3), 403-414 (2012).