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6S RNA is a highly abundant small noncoding RNA found in diverse classes of prokaryotes, including bacteria with an extremely small genome, that indicates an important role of 6S RNA in the cell. 6S RNA has a unique secondary structure which mimics DNA promoter, thus 6S RNA is able to bind RNA polymerase (RNAP) holoenzyme and inhibit transcription in case of an unfavorable environment. Moreover, DNAdependent RNAP can use 6S RNA as a template for synthesis of short pRNAs, when it needs to release from 6S RNA and proceed transcription of genes. Under normal conditions 6S RNA knockout does not affect E. coli growth, while it becomes essential for cell adaptivity to starvation and alkaline stress. Some other bacteria express 6S RNA at highest levels under standard cultivation conditions. These facts raise a number of questions, like (1) what is the mechanism of 6S RNAdependent transcription inhibition (and its retraction) in these bacteria, (2) whether 6S RNA is involved in some stress responses, and (3) what type of genes are affected. In the present work we characterized properties and functions of 6S RNAs from B. subtilis and R. sphaeroides in comparison to E. coli. It was shown that in B. subtilis 6S RNA knockouts leads to widespread changes in cellular transcriptome and proteome and results into high cell viability under alkaline conditions. For the first time, R. sphaeroides 6S RNA was characterized and osmotic and oxidative phenotypes of cells with deletion of 6S RNA gene were found. Surprisingly, decreased viability of E. coli 6S RNA knockout strain was observed in case of response to one of the stress, and some genes affected by the lack of 6S RNA under these conditions were revealed by qRTPCR. Thus, for the first time we experimentally proved a great variety of 6S RNA functions in different bacteria and its involvement into diverse cellular stress responses. This work was supported by the Russian Foundation for Basic Research (project No. 190400791).
№ | Имя | Описание | Имя файла | Размер | Добавлен |
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1. | программа конференции | programme_FEBS_2019.pdf | 1,2 МБ | 27 ноября 2019 [Daria_Elkina] | |
2. | Полный текст | тезисы | FEBS-3.pdf | 139,9 КБ | 27 ноября 2019 [Daria_Elkina] |