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Ultrafine particles released by internal combustion engines are considered to constitute a substantial part of aerosols loading in urban areas of high traffic and population (Stolcpartova et al. 2015). Campaigns including simulations and test runs of modern combustion engines offer a powerful tool for investigation of chemical composition of particulate emission in dependence on engine technology, combustion processes, fuel, operation conditions, and driving cycles (Popovicheva et al., 2015). In this study, the effects of commonly used fuels were examined on three representative engines. Blends of diesel (B0), 30% biodiesel with diesel fuel (B30), and neat biodiesel (B100) were tested in an Iveco Tector heavy-duty diesel engine operated on engine dynamometer over a World Harmonized Transient Cycle (WHTC) and steady-state conditions (1500 rpm and 30% load). Blends of 15% ethanol, 25% of n-butanol, and 25% of isobutanol with gasoline were tested in a Ford Ecoboost 1.0 direct injection gasoline engine (DISI) in a Ford Focus car, and in a Škoda 1.4 multi-point injection (MPI) engine in a Škoda Fabia car. The cars were tested on a chassis dynamometer using the Artemis driving cycle (urban, rural and highway).