ИСТИНА

Introduction. Neurodegenerative diseases, such as Parkinson’s disease, belong to the category of systemic diseases, therefore details of corresponding immunity response in different tissues of patients may be important for pathogenesis understanding. The aim of this work is to evaluate changes in the expression of innate immunity and PUFAs cascades genes, in which members are active participants of inflammatory processes, in various tissues of patients with Parkinson disease. Methods. Datasets’ search was performed using GEO and guided by the following criteria: Parkinson’s disease, Homo sapiens, RNA-sequencing, total/polyA RNA, more than 10 samples, free access, material was obtained directly from patients. As a result, 6 datasets were selected: GSE114517, GSE125239, GSE68719, GSE135036, GSE88888 and GSE128177. Salmon software (v1.0.0) was used for wicked-fast transcript quantification from RNA-seq data, taking reference human transcriptome GRCh38. The expression was summarized at the gene and transcript levels using R tximport. Age, sex and diagnosis were included in the design as covariates. Differential gene and transcript analysis were performed using DESeq2, DRIMSeq, and stageR. Resulting lists of genes were filtered by innate immunity and PUFAs cascade genes (roster from InnateDB and independently compiled list respectively). Results. Number and content of obtained lists of differentially expressed genes and differentially used transcripts were compared between tissues, paths of cascades that are key in Parkinson disease were found. Among PUFA differentially expressed genes PLA1A and cytochrome genes should be underlined. Dataset GSE68719 (Brodmann area 9) is the leader in the number of innate and PUFA differentially used transcripts. Acknowledgments. The reported study was funded by RFBR according to the research project № 19-29-01243.