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Interest is related to the possibility of receiving new information, better understanding of current information, enhanced attention to the object, information processing, comprehension of the object, learning and motivation. The aim of this study is an investigation of eye movements and EEG parameters associated with interest. 38 healthy subjects aged from 18 to 27 years (mean age 22 ± 0,4) took part in this study. Theta band power, fixation duration and amplitude of saccades are associated with interest estimate, inclusive of hesitation. These parameters might be used for the determination of cognitive-emotional state or evaluation of content, for example, educational materials.