ИСТИНА

Cnidaria is a phylum that comprises a diverse group of aquatic animals which occupy a key phylogenetic position as the sister group to all bilaterians. Hydrozoa is a rather varied group of Cnidaria of mostly colonial forms. The relatively simple organization of Hydrozoans, combined with their extreme diversity in terms of colony architectures makes them an ideal assemblage to investigate the evolution of key aspects of body plan diversification. However, only limited genomic datasets are available for hydroids, and this issue restricts the ability to engage in comparative research. Thus, our work is focused on partially filling this knowledge gap using NGS technology to generate and analyze reference transcriptomes of the two non-model marine colonial hydrozoans with different types of colony organization: Dynamena pumila (Linnaeus, 1758) with monopodial growth, and Gonothyraea loveni (Allman, 1859) with sympodial growth. For transcriptomes sequencing, we used Illumina RNA-seq data and standard protocols of assembly using rnaSPADES software. Obtained transcriptomes provide us information regarding toolkit of developmental signaling pathways such as WNT or TGF-beta and species-specific regulatory genes in hydrozoans with different body plans. Our high-quality transcriptomic data expands opportunities and opens up promising avenues for comparative evolutionary studies using in situ hybridization methods, differential gene expression analysis tools, and gene knockdown methods. This work is funded by RFBR, #20-04-00978A.