ИСТИНА

Direct enzymatic bioelectrocatalysis of pyrroloquinoline quinone dependent glucose dehydrogenase (PQQ-GDH) is exploited in glucose biosensing [1]. However, since enzyme active site is embedded inside the protein structure, various strategies for improving direct electron transfer (DET) are applied. The known systems rely on sophisticated matrixes such as graphdiyne [2], cryogels [3] etc. For industry and mass-production, on the contrary, simple, scalable, and reliable approaches are required. For example, conductive carbon black nanoparticles (CBNPs) are suitable modifiers for facilitating DET due to their biocompatibility, low cost, and large accessible electroactive area. In this work, we report on facile and scalable method of fabrication of third generation glucose biosensors based on self-made CBNPs.