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The development of high sensitive, low-cost, reliable biosensors having an excellent selectivity is a key task for medicine, environmental protection and monitoring of chemical spills. Nanomaterial for bioapplications (for instance, zinc oxide (ZnO) nanoparticles) must possess specific physical properties (e.g., optical), be tolerant to surface modification and attachment to biomolecules such as antibodies, enzymes, etc, and also non-toxic and safe. However, stability of nanoparticle dispersions in buffer solutions, which is crucially important for practical use of nanoparticles, is still an issue. Polyethyleneimine (PEI) is a promising candidate both for ZnO nanoparticle stabilization and biofunctionalization.