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Fibrosis is an ineligible outcome of the tissue response to injuries. Multipotent mesenchymal stromal cells (MSCs) play a dual role in the fibrosis development: MSCs can contribute to a myofibroblast pool therefore promoting the progression of fibrosis but they also could suppress fibrosis by producing anti-fibrotic cytokines and microRNAs. These data suggest that MSC could contain distinct subpopulations which respond differently to fibrotic stimuli. To test this hypothesis, we cultured human adipose-derived MSCs for 4 days in the presence of a key profibrotic factor TGFb on fibrotic-like decellularized ECM produced by fibroblasts and analyze cell response to fibrotic stimuli using scRNAseq.