ИСТИНА

Lipid bilayer vesicles are widely used as containers for encapsulation and delivery of bioactive compounds. Multi-liposomal complexes composed of liposomes loaded with different fillings could enable controlled delivery of the medicines in a desirable ratio. This allows to enhance drug loading and offers a simple way for formation of patient-oriented multi-functional drug formulations. In this work, we suggest a universal approach for preparation of biodegradable multi-liposomal containers through electrostatic adsorption of two- and three-component anionic liposomes on the surface of chitosan nanoparticles. Chitosan is a commercially available cationic polymer of natural origin. It is biocompatible and biodegradable. Each chitosan particle could adsorb up to 150 anionic liposomes. The size of the conjugates allows to use them as a drug carrier that is able to penetrate into the cells by passive transport mechanism. The conjugates demonstrated stability towards dissociation in physiological media. The complexes were biodegradable and eventually decomposed to 10-15 nm particles after addition of proteolytic enzymes. The degradation was accompanied by a complete release of the encapsulated drug into surrounding solution. It was revealed that multi-liposomal complexes and the products of biodegradation were non-toxic in a wide range of concentrations. The obtained results are promising in the field of drug delivery.