ИСТИНА

Development the vesicular drug carriers through the skin are prospective medical project. Lipid matrix of the outermost layer of the skin, stratum corneum (SC), is major skin barrier. Important scientific problems should be solved for the project realization: 1. Nanostructure of the stratum corneum lipid matrix and significance of individual ceramide molecules for organization of the drug diffusion road. 2. Development of stable unilamellar vesicles (ULVs) with high deformation properties. Development the methods for the ULVs characterization. Presented report relates to these two problems. Main results are: I. Significance of the ceramide 6 molecules in the creation of super-strong interaction in the SC lipid matrix have been demonstrated via neutron diffraction on the oriented model SC membranes [1,2]. II. Method of separated form factors (SFF) was developed for the characterization of the unilamellar vesicles via small-angle neutron scattering. Application of the SFF method for DMPC vesicles demonstrates the dependence of the bilayer structure and hydration on the membrane curvature [3,4]. Some pharmaceutical conclusions will be formulated based on the presented results. References: 1. M.A. Kiselev, N. Yu.Ryabova, A.M. Balagurov, S. Dante, T. Hauss, J. Zbytovska, S. Wartewig, R.H.H. Neubert. New insights into structure and hydration of stratum corneum lipid model membrane by neutron diffraction. European Biophys. J. 34 [2005] 1030–1040. 2. M.A. Kiselev. Conformation of ceramide 6 molecules and chain–flip transitions in the lipid matrix of the outermost layer of mammalian skin, the stratum corneum. Crystallography Reports 52 (2007) 525–528. 3. М.А. Kiselev, E.V. Zemlyanaya, V.K. Aswal, R.H.H. Neubert. What can we learn about the lipid vesicle structure from the small angle neutron scattering experiment? European Biophys. J. 35 [2006] 477-493. 4. M.A. Kiselev, E. V. Zemlyanaya, N.Y. Ryabova, T. Hauss, S. Dante, D. Lombardo. Water distribution function across the curved lipid bilayer: SANS study. Chemical Physics 345 (2008) 185-190.