ИСТИНА

Classic way of biocatalyst preparation included wide screening of different sources of enzymes. It is extensive and expensive way. Sequencing of genomes and development of bioinformatic approaches accelerated speed of target enzymes. Enzymes found by classic screening very often have different conditions for optimal activity compared to optimal conditions of biocatalytic process. Genetic engineering and ration design give In our laboratory we carry out systematic studies structure-function relationships nad protein design of physiologically important enzymes which are also of high practical interest. In this report we will show the most interesting examples about cloning, expression and protein design of formate dehydrogenases from different planst and moss, D-amino acids oxidase from yeasts T.variabilis and C.boidinii, penicillin acylase. This work was supported by Russian Science Foundation (grant 16-14-00043) and Russian foundation for basic research (grants 17-04-01469-а, 17-04-01487а, 17-04-01662а).