ИСТИНА

Microbial and abiotic degradation of biomacromolecules leads to formation of heterogenous supramolecular mixtures of natural organic compounds, named humic substances (HS). Activity of HS against a wide range of enveloped and non-enveloped DNA and RNA viruses allows to consider them as a promising tool against viral pathogens with no approved therapeutics. The structure and composition of HS as well as the mechanism of their action are unclear. In this study we proposed a methodology for identification of active HS components using Fourier transform ion cyclotron resonance mass-spectrometry (FTICR MS) and chemical database search. This methodology was applied for a decomposition of HS antiviral activity against tick-borne encephalitis virus (TBEV) and enteroviruses. We investigated the antiviral activity of nine HS samples against TBEV and enteroviruses and identified the formulas, to which the activity of the HS samples could be attributed. The search for these formulas in large bioactivity databases (ChEMBL, PubChemBioAssay) allowed us to find structures with relevant activity profile. On the basis of these data, we suggested the mode of action and the structures of the compounds behind HS antiviral activity.