ИСТИНА

Various authors have demonstrated that selection of vacuole-free spermatozoa for intracytoplasmic sperm injection (ICSI) leads to higher pregnancy rates and lower rates of pregnancy loss. Bartoov et al. (2001) developed the intracytoplasmic morphologically selected sperm injection (IMSI) method which included the microscope for vacuole visualization and specific morphological criteria of spermatozoa selection for ICSI. It was shown that selection of vacuole-free spermatozoa for intracytoplasmic sperm injection (ICSI) leads to higher pregnancy rates and lower rates of pregnancy loss (Berkovitz et al., 2006). The relationship between vacuolization and DNA fragmentation has also been established. However, IMSI does not provide the scientists with the possibility of visualization of the intracellular structures other than vacuoles. Our aim was developing a new microscopic method of sperm morphology evaluation, allowing us to visualize the intracellular organelles of spermatozoa.