ИСТИНА

Gene expression is a multi-level process that determines the morphological and functional characteristics of a cell. Different levels of gene expression are controlled by diverse regulatory mechanisms, from DNA epigenetic marks to post-translational modification of proteins. A significant regulatory layer is mediated by mRNA untranslated regions (5'UTR and 3'UTR), which significantly influence the mRNA translation and stability of the transcribed RNA molecules. With the advent and rapid development of massively parallel reporter assays, it became possible to profile the regulatory effects of thousands of UTRs and identify and model the sequence-level regulatory grammar with the help of deep learning. Yet, existing studies fail to properly capture and predict the UTR-dependent pattern of cell type-specific mRNA translation and stability.