ИСТИНА

Nowadays the use of natural (sugars and sugar alcohols) and artificial sweeteners is increasing. High intake of these substances can have a negative impact on human health; therefore, monitoring their levels in consumable products is essential. Usually, the determination of sweeteners is carried out in reverse-phase chromatography. However, since sugar alcohols and artificial sweeteners are hydrophilic in nature, their determination or simultaneous determination with sugars is promising by hydrophilic interaction liquid chromatography (HILIC). Applying HILIC and hydrophilic adsorbents with evaporative light-scattering detector (ELSD) can be one of the better options for simultaneous determination of sugars and sugar alcohols to achieve great retention and selectivity. The use of mixed-mode adsorbents can also ensure the retention of sweeteners including artificial, due to the implementation of various interactions, provide alternative selectivity and, finally, simultaneous separation with sugars. The work was supported by Russian Science Foundation through the grant № 20-13-00140.