ИСТИНА

Biosensors based on electrolyte-gated organic field-effect transistors (EGOFET) are very promising devices for biological research and clinical diagnostics due to their inherent advantages, such as cost-effectiveness in use and sensitivity for different analytes. However, they also have some disadvantages. One of the main problems is the difficulty or impossibility of reusing the device. Even in cases where it is possible to restore the biorecognition layer, this is associated with labor-intensive procedures that often lead to a decrease in sensitivity. Often, the biosensitivity of EGOFET is realized by modifying the gate or surface of the organic semiconductor (OS) of the transistor. In this paper, a new approach to creating a receptor layer in the composition of an EGOFET-based aptasensor using track membranes is proposed. The membrane is located on the OS surface, acting as a bioreceptor layer due to modification with a DNA aptamer. • The detection limit of this sensor is 1 105 VP/mL, which is superior to antibody-based rapid analysis test systems (1 106-4 108 VP/mL), but inferior to the PCR method (3 102-1.2 103 VP/mL); • This approach opens up the possibility of creating a reusable biosensor, which was not possible in the case of modification of the semiconductor directly with a receptor layer.