ИСТИНА

Potato virus Y (PVY) is an important plant virus and causes great yield and quality losses. An increasing number of reports reveal that in addition to their well-known roles in RNA metabolism and RNA-protein assembly, the nucleolus and Cajal bodies (CBs), both located within the cell nucleus, are involved in plant virus interactions. In particular, nucleolar (fibrillarin) and CB (coilin) proteins have been shown to interact with proteins encoded by several plant viruses. To examine whether fibrillarin and coilin play roles in PVY infection, expression of these proteins was suppressed in Nicotiana tabacum plants using transgenic (RNAi) silencing (knock-down, KD).