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The CRISPR/Cas9 system consists of clustered regulatory short palindromic repeats combined with associated genes to form a prokaryotic immune system which is able to adapt and sustain resistance against malicious plasmids and viruses. Due to addressing simplicity CRISPR/Cas9 has become very handful and popular technique to mutate genomes of various organisms (point, insertion or deletion mutations). One of the most important stages in the development of an application for genome editing using CRISPR/Cas9 is the design of guide RNAs (gRNAs). The aim of this work was to develop applications for effective cleavage of DNA sequences encoding coilin and phytoene desaturase(PDS) genes ( Solanum tuberosum).