ИСТИНА

http://www.isit2014.net Control for contaminants such as pesticides are a serious and growing problem for world as well as for the Russia and China. It is clear, that the pesticides control must be in global scale with high-throughput screening (HTS). The current methods of assays like chromatography techniques and ELISA have several limitations for HTS. Fluorescence Polarization Immunoassay (FPIA) could be the optimal method for screening and quantitative monitoring of pesticides in environmental and food samples. The FPIA is homogeneous method based on the competitive binding and the measurement of fluorescence polarization value for reaction mixture of sample (typically 10-50 µl) and immunoreagents: fluorescent-labeled antigen (tracer) and specific antibodies to analyte. The total time required for an assay is few seconds or minutes. The limit of detection for pesticides could be reached lower than typical Maximum Residue Limit (100 ng/ml) after careful optimization antibodies and tracers. The recent results for development of FPIAs for detection of carbofuran, butachlor, organophosphorus pesticides, neonicotinoid insecticides, strobilurin fungicides and dichlorophenoxyacetic acid (2,4-D) will be discussed.