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Determination of hemostatic proteins is important branch of diagnostic medicine. Diagnostic testing is often employed for selecting appropriate and optimal therapies based on the context of a patient’s genetic content or other molecular or cellular analysis. Some of the proteins in snake venom have very specific effects on protein C and X factor. But these diagnostic system are expensive. The aim of this work was selection of optimal concentrations range of extracellular protease-activator of micromycete Aspergillus ochraceus. These proteases have protein C and factor X activator activity. Efficiency of isolated protease for protein C and X factor determination was compared with commercial preparations - Protac® and RVV-X® from snakes’ venom in different human plasmas. It was demonstrated the same results with specific activity proteinases of Aspergillus ochraceus and with Protac® and RVV-X®. Similar experiments were conducted in the presence of factor X deficient plasma and protein C deficient plasma. It was found that concentration of protein C is identical with Protac® (32,7% ± 5%) diagnostic system and specific activity proteinases of Aspergillus ochraceus (31,5% ± 5%). Uniform diagnostics interval was detected with RVV-X® diagnostic system (36,6% ± 4%) and proteinases of Aspergillus ochraceus (37,4% ± 4%). To sum up, proteases produced by Aspergillus ochraceus are very perspective for protein C and X factor diagnostics. This method may be cheaper and easier than another methodics.