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The possibility of the mixing layer height determination by use of the acoustic remote sensing is discussed. The short review of known methods is presented. A new method is to determine separately two different mixing layer heights: the “upper” height in stable conditions and the “lower” one which indicates unstable or weak-stable stratification below a bottom of elevated inversion. According to this idea the diurnal courses of both mixing layer heights were calculated on a base of long-term sodar observations in Moscow for period of almost 15 years.