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During last couple decades in Russia an interest in the electron-beam sterilization technology has been significantly renewed. The electron beam irradiation occurs at electron energies in the range from 3 up to 10 MeV with dose of 30 kGy. A special research interest is an exploring the possibility to reduce electron energy and dose characteristics upon foods irradiation. The aqueous suspensions with Escherichia coli and Staphylococcus aureus have been used as the research objects. Whole treatment process has been carried out on an industrial electron accelerator UELV-10-10-C-70 located in the A.N. Frumkin Institute of Physical chemistry and Electrochemistry at the Russian Academy of Sciences. Beforehand partially filled and sealed 10 ml vials containing the sample suspensions with microorganisms have been installed on an accelerator line in two different positions: vertical and horizontal. The samples were irradiated with doses of 3, 5, 7 and 10 kGy. Microbiological investigations of irradiated objects have been carried out in accordance with the Russian State Standards 30726-2011 and GOST 10444.2-94 by the most probable number of colony forming units method. As an investigative result, the microorganism radioresistance index D10 has been determined. It makes it possible to evaluate amount of these microorganism types upon contaminated foodstuffs irradiation. It has been established that during exposure the horizontal position of the vials was more effectively. It may be explained due to simultaneous influence of two different though interrelated reasons: the direct radiation and radiation-induced ozone.