Dried-Reswollen Immobilized Biocatalysts for Detoxification of Organophosphorous Compounds in the Flow Systemsстатья
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Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Аннотация:New immobilized biocatalysts based on polypeptides containing N- or C-terminal polyhistidine sequences and possessing organophosphorus hydrolase activity were investigated for detoxification of organophosphorous neurotoxic compounds in the flow systems. The biocatalysts were revealed to have a high catalytic activity within wide pH and temperature ranges 7.5–12.5 °C and 15–65 °C, respectively. The immobilized biocatalysts can be dried and reswollen before use with 92–93% catalytic activity remaining after drying and rehydration procedures. The half-lives of the biocatalysts under wet and dry storage conditions were 420 and 540 days, respectively.