Hypoacylated LPS from Foodborne Pathogen Campylobacter jejuni Induces Moderate TLR4-Mediated Inflammatory Response in Murine Macrophages

Korneev, K.V.; Kondakova, A.N.; Sviriaeva, E.N.; Mitkin, N.A.; Palmigiano, A.; Kruglov, A.A.; Telegin, G.B.; Drutskaya, M.S.; Sturiale, L.; Garozzo, D.; Nedospasov, S.A.; Knirel, Y.A.; Kuprash, D.V.

Статья опубликована в высокорейтинговом журнале

Информация о цитировании статьи получена из Web of Science, Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 20 марта 2018 г.

Авторы: Korneev Kirill V., Kondakova Anna N., Sviriaeva Ekaterina N., Mitkin Nikita A., Palmigiano Angelo, Kruglov Andrey A., Telegin Georgy B., Drutskaya Marina S., Sturiale Luisa, Garozzo Domenico, Nedospasov Sergei A., Knirel Yuriy A., Kuprash Dmitry V.
Журнал: Frontiers in Cellular and Infection Microbiology
Том: 8
Номер: 58
Год издания: 2018
Издательство: Frontiers Media S.A.
Местоположение издательства: Lausanne, Switzerland
Первая страница: 1
Последняя страница: 10
DOI: 10.3389/fcimb.2018.00058
Аннотация: Toll-like receptor 4 (TLR4) initiates immune response against Gram-negative bacteria upon specific recognition of lipid A moiety of lipopolysaccharide (LPS), the major component of their cell wall. Some natural differences between LPS variants in their ability to interact with TLR4 may lead to either insufficient activation that may not prevent bacterial growth, or excessive activation which may lead to septic shock. In this study we evaluated the biological activity of LPS isolated from pathogenic strain of Campylobacter jejuni, the most widespread bacterial cause of foodborne diarrhea in humans. With the help of hydrophobic chromatography and MALDI-TOF mass spectrometry we showed that LPS from a C. jejuni strain O2A consists of both hexaacyl and tetraacyl forms. Since such hypoacylation can result in a reduced immune response in humans, we assessed the activity of LPS from C. jejuni in mouse macrophages by measuring its capacity to activate TLR4-mediated proinflammatory cytokine and chemokine production, as well as NFkB-dependent reporter gene transcription. Our data support the hypothesis that LPS acylation correlates with its bioactivity.
Добавил в систему: Корнеев Кирилл Викторович

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Hypoacylated LPS from Foodborne Pathogen Campylobacter jejuni Induces Moderate TLR4-Mediated Inflammatory Response in Murine Macrophagesстатья Исследовательская статья

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