Use of piezoelectric immunosensors for detection of interferon- gamma interaction with specific antibodies in the presence of released active forms of antibodies to interferon-gamma.Sensors

Don, E.; Pokhil, S.; Barykina, D.; Nikiforova, M.; Shulga, D.; Borshcheva, A.; Tarasov, S.; Epstein, O.; Farafonova, O.; Ermolaeva, T.

Статья опубликована в высокорейтинговом журнале

Информация о цитировании статьи получена из Web of Science, Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 20 марта 2018 г.

Авторы: Don E., Pokhil S., Barykina D., Nikiforova M., Shulga D., Borshcheva A., Tarasov S., Epstein O., Farafonova O., Ermolaeva T.
Журнал: Sensors
Том: 16
Номер: 1
Год издания: 2016
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Первая страница: 96
Последняя страница: 108
DOI: 10.3390/s16010096
Аннотация: In preliminary ELISA studies where released-active forms (RAF) of antibodies (Abs) to interferon-gamma (IFNg) were added to the antigen-antibody system, a statistically significant difference in absorbance signals obtained in their presence in comparison to placebo was observed. A piezoelectric immunosensor assay was developed to support these data and investigate the effects of RAF Abs to IFNg on the specific interaction between Abs to IFNg and IFNg. The experimental conditions were designed and optimal electrode coating, detection circumstances and suitable chaotropic agents for electrode regeneration were selected. The developed technique was found to provide high repeatability, intermediate precision and specificity. The difference between the analytical signals of RAF Ab samples and those of the placebo was up to 50.8%, whereas the difference between non-specific controls and the placebo was within 5%–6%. Thus, the piezoelectric immunosensor as well as ELISA has the potential to be used for detecting the effects of RAF Abs to IFNg on the antigen-antibody interaction, which might be the result of RAF’s ability to modify the affinity of IFNg to specific/related Abs.
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Use of piezoelectric immunosensors for detection of interferon- gamma interaction with specific antibodies in the presence of released active forms of antibodies to interferon-gamma.Sensorsстатья

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