Expansions, diversification, and interindividual copy number variations of AID/APOBEC family cytidine deaminase genes in lampreysстатья
Статья опубликована в высокорейтинговом журнале
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Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 10 августа 2018 г.
Авторы:
Holland Stephen J.,
Berghuis Lesley M.,
King Justin J.,
Iyer Lakshminarayan M.,
Katarzyna Sikora,
Heather Fifield,
Sarah Peter,
Quinlan Emma M.,
Fumiaki Sugahara,
Prashant Shingate,
Inês Trancoso,
Norimasa Iwanami,
Elena Temereva,
Christine Strohmeier,
Shigeru Kuratani,
Byrappa Venkatesh,
Guillaume Evanno,
Aravind L.,
Michael Schorpp,
Mani Larijani,
Thomas Boehm
Аннотация:Cytidine deaminases of the AID/APOBEC family catalyze C-to-U nucleotide transitions in mRNA or DNA. Members of the APOBEC3 branch are involved in antiviral defense, whereas AID contributes to diversification of antibody repertoires in jawed vertebrates via somatic hypermutation, gene conversion, and class switch recombination. In the extant jawless vertebrate, the lamprey, two members of the AID/APOBEC family are implicated in the generation of somatic diversity of the variable lymphocyte receptors (VLRs). Expression studies linked CDA1 and CDA2 genes to the assembly of VLRA/C genes in T-like cells and the VLRB genes in B-like cells, respectively. Here, we identify and characterize several CDA1-like genes in the larvae of different lamprey species and demonstrate that these encode active cytidine deaminases. Structural comparisons of the CDA1 variants highlighted substantial differences in surface charge; this observation is supported by our finding that the enzymes require different conditions and substrates for optimal activity in vitro. Strikingly, we also found that the number of CDA-like genes present in individuals of the same species is variable. Nevertheless, irrespective of the number of different CDA1-like genes present, all lamprey larvae have at least one functional CDA1-related gene encoding an enzyme with predicted structural and chemical features generally comparable to jawed vertebrate AID. Our findings suggest that, similar to APOBEC3 branch expansion in jawed vertebrates, the AID/APOBEC family has undergone substantial diversification in lamprey, possibly indicative of multiple distinct biological roles.