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Stabilizing the Central Part of Tropomyosin Increases the Bending Stiffness of the Thin Filamentстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 19 сентября 2015 г.
- Авторы: Nabiev SR, Ovsyannikov DA, Kopylova GV, Shchepkin DV, Matyushenko A., Koubassova NA, Levitsky DI, Tsaturyan AK, Bershitsky SY
- Журнал: Biophysical Journal
- Том: 109
- Номер: 2
- Год издания: 2015
- Издательство: Biophysical Society
- Местоположение издательства: United States
- Первая страница: 373
- Последняя страница: 379
- DOI: 10.1016/j.bpj.2015.06.006
- Аннотация: A two-beam optical trap was used to measure the bending stiffness of F-actin and reconstructed thin filaments. A dumbbell was formed by a filament segment attached to two beads that were held in the two optical traps. One trap was static and held a bead used as a force transducer, whereas an acoustooptical deflector moved the beam holding the second bead, causing stretch of the dumbbell. The distance between the beads was measured using image analysis of micrographs. An exact solution to the problem of bending of an elastic filament attached to two beads and subjected to a stretch was used for data analysis. Substitution of noncanonical residues in the central part of tropomyosin with canonical ones, G126R and D137L, and especially their combination, caused an increase in the bending stiffness of the thin filaments. The data confirm that the effect of these mutations on the regulation of actin-myosin interactions may be caused by an increase in tropomyosin stiffness.
- Добавил в систему: Цатурян Андрей Кимович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | Nabiev_bj_2015.pdf | 524,6 КБ | 21 сентября 2016 [n.koubassova] |