Use of absorption spectra and their second-order derivative to quantify degradation of lignohumate by filamentous fungi

Fedoseeva, E.; Khundzhua, D.; Terekhova, V.; Patsaeva, S.

Авторы: Fedoseeva Elena, Khundzhua Daria, Terekhova Vera, Patsaeva Svetlana
Сборник: Proceedings Volume 10614 XIII International Conference on Atomic and Molecular Pulsed Llasers. 10-15 september 2017
Серия: Proceedings of SPIE
Том: 10614
Год издания: 2018
Издательство: SPIE, the International Society for Optical Engineering
Местоположение издательства: Bellingham, WA, United States
Первая страница: 106142B-1
Последняя страница: 106142B-7
DOI: 10.1117/12.2303617
Аннотация: Soil fungi are actively involved in the processes of humic substances synthesis, transformation and mineralization due to production of extracellular nonspecific oxidative enzymes. The work was aimed to evaluate using spectral methods transformation dynamics for the humic product (HP) from lignosulfonate (HPligno) by filamentous soil fungal cultures Alternaria alternata and Trichoderma harzianum. Experiments showed that direct spectroscopic study of HPligno introduced into the nutrient medium and its transformation during fungal growth is challenging due to strong absorption of light by nutrient medium, development of absorbing fungal metabolites, partial utilization and destruction of HP by fungi and therefore due to the need to register tiny changes in overlapping bands. To accomplish that task we proposed a novel algorithm for processing the absorption spectra, which has not previously been used to study fungal cultures. We calculated the second-order derivative in respect to wavelength for absorption spectra measured during fungal growth and found characteristic "patterns" for introduced HP: a maximum at 270-285 and a minimum within 290-300 nm. The spectral index determined from amplitudes in the second-order derivative spectrum reflects the relative content of HP in the nutrient medium in presence of other absorbing components. We resume that two fungal strains utilized HPligno in the 0,02 and 0,1% concentrations better at 30 g/L sucrose than at 3 g/L in the medium. Thus the second-order differentiated absorption spectra helped to quantify degradation of the HPligno during fungal growth. Keywords: Absorbance spectra, second derivative spectroscopy, optical indices, humic substances, fungal chromophores, Alternaria alternatа, Trichoderma harzianum.
Добавил в систему: Терехова Вера Александровна

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Use of absorption spectra and their second-order derivative to quantify degradation of lignohumate by filamentous fungiстатья

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