Quantification of Rat Brain Amino Acids: Analysis of the Data Consistencyстатья
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Дата последнего поиска статьи во внешних источниках: 26 августа 2016 г.
Аннотация:BACKGROUND: Tissue amino acids are important markers to characterize (patho)physiology. As neurotransmitters or their precursors, they have special significance in brain. However, mass spectrometry (MS)-based quantifications of brain amino acids are rare and inconsistent.
METHODS: To reveal efficient procedures optimal for brain research purposes, free amino acids in the same extracts of rat cerebellum were quantified by published assays employing hybrid triple quadrupole-linear ion trap (QTRAP) MS or amino acid analyzer with ninhydrin derivatization.
RESULTS: Levels of four amino acids out of 14 quantified by the two methods were established to be perturbed in the QTRAP MS assay, with their ninhydrin-based quantification in accordance with the coinciding published quantifications by non-MS approaches. The overestimation of Glu and Lys and underestimation of Gly and Tyr in brain extracts by QTRAP MS suggest interference of the co-eluting compounds and ion suppression effects, correspondingly. The MS-inherent artifacts presumably contribute to inconsistency of published MS data on the brain amino acid content, as our analysis of different studies indicated that the variability of the MS data greatly exceeds the biological variability.
CONCLUSION: Evaluation of potential artifacts of the MS-based quantification of brain amino acids using the ninhydrin detection as a reference method, provides a solution for reliable quantification of the artifact-resistant amino acids, pointing to relatively good performance of the time- and cost-efficient QTRAP MS procedure employed.