Fiber-optic control and thermometry of single-cell thermosensation logic

Fedotov, I.V.; Safronov, N.A.; Ermakova, Y.G.; Matlashov, M.E.; Sidorov-Biryukov, D.A.; Fedotov, A.B.; Belousov, V.V.; Zheltikov, A.M.

Статья опубликована в высокорейтинговом журнале

Информация о цитировании статьи получена из Web of Science, Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 17 декабря 2015 г.

Авторы: Fedotov I.V., Safronov N.A., Ermakova Yu G., Matlashov M.E., Sidorov-Biryukov D.A., Fedotov A.B., Belousov V.V., Zheltikov A.M.
Журнал: Scientific reports
Том: 5
Год издания: 2015
Издательство: Nature Publishing Group
Местоположение издательства: United Kingdom
Первая страница: 15737
DOI: 10.1038/srep15737
Аннотация: Thermal activation of transient receptor potential (TRP) cation channels is one of the most striking examples of temperature-controlled processes in cell biology. As the evidence indicating the fundamental role of such processes in thermosensation builds at a fast pace, adequately accurate tools that would allow heat receptor logic behind thermosensation to be examined on a single-cell level are in great demand. Here, we demonstrate a specifically designed fiber-optic probe that enables thermal activation with simultaneous online thermometry of individual cells expressing genetically encoded TRP channels. This probe integrates a fiber-optic tract for the delivery of laser light with a two-wire microwave transmission line. A diamond microcrystal fixed on the fiber tip is heated by laser radiation transmitted through the fiber, providing a local heating of a cell culture, enabling a well-controlled TRP-assisted thermal activation of cells. Online local temperature measurements are performed by using the temperature-dependent frequency shift of optically detected magnetic resonance, induced by coupling the microwave field, delivered by the microwave transmission line, to nitrogen—vacancy centers in the diamond microcrystal. Activation of TRP channels is verified by using genetically encoded fluorescence indicators, visualizing an increase in the calcium flow through activated TRP channels.
Добавил в систему: Федотов Андрей Борисович

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Fiber-optic control and thermometry of single-cell thermosensation logicстатья

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