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Calcium ions inhibit reduction of heme a in bovine cytochrome c oxidaseстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 30 марта 2016 г.
- Авторы: Dyuba AV, Vygodina T., Azarkina N., Konstantinov AA
- Журнал: FEBS Letters
- Том: 589
- Год издания: 2015
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 3853
- Последняя страница: 3858
- DOI: 10.1016/j.febslet.2015.11.023
- Аннотация: The effect of Ca2+ on the rate of heme a reduction by dithionite and hexaammineruthenium (RuAm) was studied in the cyanide-complexed bovine cytochrome oxidase (CcO). The rate of heme a reduction is proportional to RuAm concentration below 300 μM with kv of 0.53×106 M-1s-1. Ca2+ inhibits the rate of heme a reduction by dithionite by ∼25%. As the reaction speeds up with increased concentrations of RuAm, the inhibition by Ca2+ disappears. The inhibition of heme a reduction may contribute to recently described partial inhibition of CcO by Ca2+ in the enzymatic assays. The inhibitory effect of Ca2+ on heme a reduction indicates that ET through heme a may be coupled to proton movement in the exit part of the proton channel H.
- Добавил в систему: Константинов Александр Александрович