New nanocomposites for SERS studies of living cells and mitochondriaстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
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Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 26 сентября 2016 г.
Аннотация:A great enhancement of Raman scattering (SERS) from heme - containing submembrane biomolecules inside intact erythrocytes and functional mitochondria is demonstrated for the first time using silver – silica beads prepared by a new method of aerosol pyrolysis with aqueous diamminesilver (I) hydroxide as a unique source of plasmonic nanoparticles for SiO2 microsheres. The recorded SERS spectra revealed a set of characteristic peaks at 750, 1127, 1170, 1371, 1565, 1585 and 1638 cm-1 resulting from the normal group vibrations of pyrrol rings, methine bridges and side radicals in the heme molecules. The SERS spectra of functional mitochondria were sensitive to the activity of the mitochondrial electron transport chain thus making the method to be a novel label-free approach to monitor the redox state and conformation of cytochromes in their natural cell environment. The developed nanocomposites are highly suitable for the analysis of bilogical objects due to their robust prepared scheme, superior spatial and temporal signal reproducibility preserved for a period of at least one year.