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Development of a simple, rapid and high-throughput fluorescence polarization immunoassay for glycocholic acid in human urineстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 4 сентября 2018 г.
- Авторы: Qiyi He, Cui Xiping, Shen Ding, Chen Yingshan, Jiang Zhengyun, Rui Lv, Eremin Sergei A., Zhao Suqing
- Журнал: Journal of Pharmaceutical and Biomedical Analysis
- Том: 158
- Год издания: 2018
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 431
- Последняя страница: 437
- DOI: 10.1016/j.jpba.2018.06.023
- Аннотация: In this paper, a simple, rapid and high-throughput fluorescence polarization immunoassay (FPIA) based onpolyclonal antibodies (PAb) is described for the determination of glycocholic acid (GCA) in human urine.Three fluorescein-labeled GCA (tracers) with different structures and spacer bridges were synthesized andpurified by thin-layer chromatography (TLC). The structure effect of tracers on the assay was investigatedand the sensitivity of best tracer in the optimized FPIA demonstrated an IC50value of 306 ng/mL. Theworking range of FPIA was 36 - 2 600 ng/mL and the limit of detection (LOD) was 9 ng/mL. The developedFPIA was time-saving that could be completed within 10 min. Human urine samples spiked with GCA wereanalyzed by this method, followed by confirmation with commercial enzyme immunoassay analysis (EIA).Excellent recoveries and correlation between these two methods were observed (R2= 0.996), suggestingthe developed FPIA could be applied to screening of GCA in human urine samples without complicated cleanup.
- Добавил в систему: Еремин Сергей Александрович
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