2′-Fluoronucleotides as substrates of viral replicative polymerasesстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 11 декабря 2015 г.
Аннотация:Substrate specificities of three viral replicative polymerases of different origins (HIV reverse transcriptase, hepatitis C virus RNA polymerase, and herpes virus DNA polymerase) towards 2'F-NTP were studied. Activated DNA, polyA-oligoU(6), and (2'F-A)(20)-oligoU(6) complexes were used as templates. It was shown that all DNA polymerases studied incorporate 2'F-NMP into the 3'-end of primer-template complexes. HIV reverse transcriptase and herpes virus DNA polymerase can elongate synthesis with both dNTP and 2'F-NTP. Homopolymer (2'F-A)(20) can serve as a template for the polymerization of UTP as well as 2'F-UTP catalyzed by hepatitis C virus polymerase although with the efficacy about five-to tenfold lower compared to the natural primer-template complex. The pyrophosphorolysis reaction of 2'F-CMP residue on the 3'-end of the primer catalyzed with HIV reverse transcriptase proceeds with an efficiency that is two orders of magnitude less compared to the natural dNMP residues in the same system.